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Ulex Europaeus Lectin 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-13922

Novus Biologicals, part of Bio-Techne
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NB110-13922
NB110-13922-0.05ml

Key Product Details

Species Reactivity

Validated:

Plant

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Specifications

Immunogen

Anti-Ulex Europaeus-I (UEA-I) Lectin is developed in rabbit using purified Ulex Europaeus-I lectin from gorse seeds as the immunogen.

Specificity

The antibody may be used for studies of the vascular structures. It reacts specifically with UEA-I bound to human endothelial cells of normal and neoplastic blood and lymphatic vessels. Its uniform reaction with vessels enables differentation between vessels and artificial slits in the tissue.

Marker

Endothelial Cells

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ulex Europaeus Lectin 1 Antibody

Immunohistochemistry: Ulex Europaeus Lectin 1 Antibody [NB110-13922]

Immunohistochemistry: Ulex Europaeus Lectin 1 Antibody [NB110-13922]

Immunohistochemistry: Ulex Europaeus Lectin 1 Antibody [NB110-13922] - Formalin fixed, paraffin-embedded Human tonsil tissue sections were stained with 1:1000 Anti-Lectin, Ulex europaeus-I antibody produced in Rabbit followed by 1:30 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat.

Applications for Ulex Europaeus Lectin 1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Immunohistochemistry-Frozen

1:500

Immunohistochemistry-Paraffin

1:500
Application Notes
Lectin isolated from Ulex europaeus I (NBP2-42700) is specific for alpha-L-fucose-containing glycocompounds which (in combination with NB110-13922) makes it a specific marker for vascular endothelial cells and tumors derived from them. For immunoassays on animal tissues, Ulex Europaeus Lectin 1 Protein (NBP2-42700) would be a required product alongwith NB110-13922. Technically, NBP2-42700 will bind the carbohydrate moiety on vascular endothelial cells as first primary reagent and then NB110-13922 will bind to Ulex Europaeus-1 lectin (NBP2-42700) in the primary antibody incubation step (See product specific IHC-P Protocol).

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera, delipidized

Preservative

0.09% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ulex Europaeus Lectin 1

Lectins are highly specific carbohydrate binding proteins useful in carbohydrate and cell membrane studies, as well as cell agglutination and typing techniques. Anti-Ulex Europaeus-I (UEA-I) Lectin is developed in rabbit using purified Ulex Europaeus-I lectin from gorse seeds as the immunogen. Ulex Europaeus Lectin I is a 46 kDa glycoprotein known to interact with alpha-I fucosyl residues in oligosaccharides present on the membranes of human blood group O erythrocytes, human endothelial cells and a variety of human and animal epithelial cells.

Alternate Names

Gorse or Furze Lectin, UEA-1; H(O) lectin 1; Anti H(O) lectin I

Additional Ulex Europaeus Lectin 1 Products

Product Documents for Ulex Europaeus Lectin 1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Ulex Europaeus Lectin 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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