TBX15 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-49036

Novus Biologicals, part of Bio-Techne
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NBP2-49036-25ul
NBP2-49036
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TBX15 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: CSPPTFHLAPNTFNVGCRESQLCNLNLSDYPPCARSNMAALQSYPGLSDSGYNRLQSGTTSATQPSETFMPQRTPSLISGIPTPPSLPGNSKMEAYGGQLGSFPTSQFQYVMQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TBX15 Antibody - BSA Free

Western Blot: TBX15 Antibody [NBP2-49036]

Western Blot: TBX15 Antibody [NBP2-49036]

Western Blot: TBX15 Antibody [NBP2-49036] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
Lane 4: Human plasma (IgG/HSA depleted)
Immunohistochemistry-Paraffin: TBX15 Antibody [NBP2-49036]

Immunohistochemistry-Paraffin: TBX15 Antibody [NBP2-49036]

Immunohistochemistry-Paraffin: TBX15 Antibody [NBP2-49036] - Staining of human cerebral cortex shows moderate nuclear positivity in a subset of neurons.
TBX15 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: TBX15 Antibody - BSA Free [NBP2-49036]

Chromatin Immunoprecipitation-exo-Seq: TBX15 Antibody - BSA Free [NBP2-49036]

ChIP-Exo-Seq composite graph for Anti-TBX15 (NBP2-49036) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for TBX15 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunohistochemistry

1:50 -1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TBX15

The T-box (TBX) motif is present in a family of genes whose structural features and expression patterns support their involvement in developmental gene regulation. The TBX gene family are largely conserved throughout metazoan evolution, and these genes code for putative transcription factors that share a uniquely defining DNA-binding domain. TBX genes are a family of developmental regulators with more than 20 members recently identified in invertebrates and vertebrates. Mutations in TBX genes are associated with the onset of several human diseases. Our understanding of functional mechanisms of TBX products has come mainly from the prototypical T/Brachyury, which is a transcription activator. The TBX genes constitute a family of transcriptional regulatory genes that are implicated in a variety of developmental processes ranging from the formation of germ layers to the organizational patterning of the central nervous system.

Alternate Names

T-box 14, T-box 15, T-box protein 14, T-box protein 15, T-box transcription factor TBX14, T-box transcription factor TBX15, TBX14

Gene Symbol

TBX15

Additional TBX15 Products

Product Documents for TBX15 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TBX15 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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