Sphingosine Kinase 1/SPHK1 [p Ser225] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23421

Novus Biologicals, part of Bio-Techne
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NBP3-23421
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Sphingosine Kinase 1/SPHK1 Antibodies »

Product Specifications

Immunogen

Phospho-Sphingosine Kinase 1/SPHK1 (Ser-225) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding serine 225 in human Sphingosine Kinase 1/SPHK1. This sequence has 4 amino acid differences from mouse and 5 from rat Sphingosine Kinase 1/SPHK1, and is not homologous to sequences in SK2. The conserved site in rat and mouse Sphingosine Kinase 1/SPHK1 is at serine 224. [UniProt# Q9NYA1]

Modification

p Ser225

Specificity

This antibody was cross-adsorbed to non-specific phosphoserine and unphosphorylated Sphingosine Kinase 1/SPHK1 (Ser-225) peptide before affinity purification using phospho-Sphingosine Kinase 1/SPHK1 (Ser-225) peptide. The purified antibody detects a 47 kDa full-length recombinant human Sphingosine Kinase 1/SPHK1 protein phosphorylated with ERK2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for Sphingosine Kinase 1/SPHK1 [p Ser225] Antibody

Sphingosine Kinase 1/SPHK1 [p Ser225] Antibody

Western Blot: Sphingosine Kinase 1/SPHK1 [p Ser225] Antibody [NBP3-23421] -

Western Blot: Sphingosine Kinase 1/SPHK1 [p Ser225] Antibody [NBP3-23421] - Western blot image of recombinant his-tagged human Sphingosine Kinase 1/SPHK1 protein that was phosphorylated with ERK-2. Blots were probed with anti-Sphingosine Kinase 1/SPHK1 (Central Region) (NBP3-23415; lanes 1-3) and anti-Sphingosine Kinase 1/SPHK1 (Ser-225) (NBP3-23421; lanes 4-6). Both antibodies were used in the presence of no peptide (lanes 1 & 4), phospho-Sphingosine Kinase 1/SPHK1 (Ser-225) peptide (lanes 2 & 5), or unphosphorylated Sphingosine Kinase 1/SPHK1 (Ser-225) peptide (lanes 3 & 6).
Sphingosine Kinase 1/SPHK1 [p Ser225] Antibody

Western Blot: Sphingosine Kinase 1/SPHK1 [p Ser225] Antibody [NBP3-23421] -

Western Blot: Sphingosine Kinase 1/SPHK1 [p Ser225] Antibody [NBP3-23421] - Western blot of HeLa stimulated with calyculin A (lanes 1-4). The blots were untreated (lane 1 & 3) or treated with lambda phosphatase (lane 2 & 4), then probed with anti-Sphingosine Kinase 1/SPHK1 (Central region) NBP3-23415 (lanes 1 & 2) or anti-Sphingosine Kinase 1/SPHK1 (Ser-225) NBP3-23421 (lanes 3 & 4).

Applications for Sphingosine Kinase 1/SPHK1 [p Ser225] Antibody

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Sphingosine Kinase 1/SPHK1

Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.[supplied by OMIM]

Alternate Names

SK1, SPK1

Gene Symbol

SPHK1

Additional Sphingosine Kinase 1/SPHK1 Products

Product Documents for Sphingosine Kinase 1/SPHK1 [p Ser225] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Sphingosine Kinase 1/SPHK1 [p Ser225] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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