SMARCA5/SNF2H Antibody (6H8I7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16216
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 6H8I7 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 953-1052 of human SMARCA5/SNF2H (O60264). RFLICMLHKLGFDKENVYDELRQCIRNSPQFRFDWFLKSRTAMELQRRCNTLITLIERENMELEEKEKAEKKKRGPKPSTQKRKMDGAPDGRGRKKKLKL
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit SMARCA5/SNF2H Antibody (6H8I7) (NBP3-16216) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for SMARCA5/SNF2H Antibody (6H8I7)
![Western Blot: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216]](https://resources.bio-techne.com/images/products/SMARCA5-SNF2H-Antibody-6H8I7-Western-Blot-NBP3-16216-img0005.jpg "Western Blot: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216]")
Western Blot: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216]
Western Blot: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216] - Western blot analysis of extracts of Mouse thymus, using SMARCA5/SMARCA5/SNF2H Rabbit mAb (NBP3-16216) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.![Western Blot: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216]](https://resources.bio-techne.com/images/products/SMARCA5-SNF2H-Antibody-6H8I7-Western-Blot-NBP3-16216-img0004.jpg "Western Blot: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216]")
Western Blot: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216]
Western Blot: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216] - Western blot analysis of extracts of various cell lines, using SMARCA5/SMARCA5/SNF2H Rabbit mAb (NBP3-16216) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216] -
Immunocytochemistry/ Immunofluorescence: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216] - Confocal imaging of Hep G2 cells using SMARCA5/SNF2H Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.Applications for SMARCA5/SNF2H Antibody (6H8I7)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SMARCA5/SNF2H
Long Name
SWI/SNF-related, Matrix-associated, Actin-dependent Regulator of Chromatin, Subfamily A, Member 5
Alternate Names
ISWI, SNF2H, WCRF135
Gene Symbol
SMARCA5
Additional SMARCA5/SNF2H Products
Product Documents for SMARCA5/SNF2H Antibody (6H8I7)
Product Specific Notices for SMARCA5/SNF2H Antibody (6H8I7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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