SF2 Antibody (24GB2255)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50620

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NBP3-50620-100ul
NBP3-50620-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB2255

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SF2 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human SF2 (Uniprot: Q07955)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SF2 Antibody (24GB2255)

SF2 Antibody (24GB2255)

Immunocytochemistry/ Immunofluorescence: SF2 Antibody (24GB2255)[NBP3-50620] -

Immunocytochemical staining of HepG2 cells with SF2 antibody (1:1,000) . Nuclei were stained blue with DAPI; SF2 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
SF2 Antibody (24GB2255)

Flow Cytometry: SF2 Antibody (24GB2255)[NBP3-50620] -

Flow cytometric analysis of SF2expression in HepG2 cells using SF2 antibody (1:2,000). Green, isotype control; red, SRSF1.
SF2 Antibody (24GB2255)

Western Blot: SF2 Antibody (24GB2255)[NBP3-50620] -

Western blotting analysis using SF2 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for SF2 Antibody (24GB2255)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SF2

SF2 encodes a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

Alternative-splicing factor 1, ASFpre-mRNA-splicing factor SF2, P33 subunit, MGC5228, serine/arginine-rich splicing factor 1, SF2FLJ53078, SF2p33, SFRS1splicing factor 2, Splicing factor, arginine/serine-rich 1ASF-1, SR splicing factor 1, SRp30a

Gene Symbol

SRSF1

Additional SF2 Products

Product Documents for SF2 Antibody (24GB2255)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SF2 Antibody (24GB2255)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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