S100A4 Antibody (CL0237) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43987

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0237

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

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Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P26447, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse S100A4 Antibody (CL0237) - Azide and BSA Free (NBP2-52890) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for S100A4 Antibody (CL0237) - Azide and BSA Free

S100A4 Antibody (CL0237) - Azide and BSA Free Western Blot: S100A4 Antibody [NBP3-43987]

Western Blot: S100A4 Antibody [NBP3-43987]

Lane 1: Marker [kDa]
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: S100A4 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
S100A4 Antibody (CL0237) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: S100A4 Antibody [NBP3-43987]

Immunocytochemistry/ Immunofluorescence: S100A4 Antibody [NBP3-43987]

Staining of A549 cells using the Anti-S100A4 monoclonal antibody) .
S100A4 Antibody (CL0237) - Azide and BSA Free Immunohistochemistry-Paraffin: S100A4 Antibody [NBP3-43987]

Immunohistochemistry-Paraffin: S100A4 Antibody [NBP3-43987]

Analysis in human tonsil and pancreas tissues using NBP3-43987 antibody. Corresponding S100A4 RNA-seq data are presented for the same tissues.
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Applications for S100A4 Antibody (CL0237) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: S100A4

S100A4 is encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Long Name

S100 Calcium Binding Protein A4

Alternate Names

18A2, 42A, CAPL, MTS1, P9KA, PEL98

Gene Symbol

S100A4

Additional S100A4 Products

Product Documents for S100A4 Antibody (CL0237) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for S100A4 Antibody (CL0237) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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