PTH1R/PTHR1 Antibody (4D2)

Immunocytochemistry/ Immunofluorescence: PTH1R/PTHR1 Antibody (4D2) - BSA Free [NBP1-51640]

Immunocytochemistry/Immunofluorescence: PTH1R/PTHR1 Antibody (4D2) [NBP1-51640] - Analysis of SK-BR-3 cells using PTH1R mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Immunocytochemistry/ Immunofluorescence: PTH1R/PTHR1 Antibody (4D2) - BSA Free [NBP1-51640] -

Immunocytochemistry/ Immunofluorescence: PTH1R/PTHR1 Antibody (4D2) - BSA Free [NBP1-51640] - Generation of functional kidney spheroids using immortalized human primary cells. (a) Quantitative RT-PCR analysis of kidney-specific segment markers in hKidS compared with 2D controls (2D CTL) & human kidney lysates (hKidney). n = 8 for 2D CTL & hKidS & n = 3 for hKidney. (b) Western blotting analysis for renal multi-lineage marker in mouse kidney spheroids compared to 2D CTL & hKidney. The grouping of gels/blots cropped from different parts of the same gel. (c) Representative images for cell polarity of Zo-1, an apical membrane, & Na/K ATPase, a basolateral membrane marker in hKidS. Scale bar, 10 μm. (d) Representative images of human kidney spheroids (hKidS) at day 5 of maturation stained for the kidney-specific markers LTL, PNA, AQP2, & Nephrin. Human kidney was used as positive control for immunostaining of hKidS. (e) Responses to parathyroid hormone (PTH) of hKidS compared with 3D control spheroids (without maturation factors, CTL) & 2D cells. (f) GGT activity in hKidS compared with 3D CTL. (g) Temperature-dependent albumin-fluorescein uptake of hKidS compared to 3D CTL. n = 10 for 3D CTL & hKidS. (h,i) Relative mRNA expression of apical (h) & basolateral (i) transporters in hKidS compared to 2D cultured cells, hPTECs spheroids generated using the same protocol for hKidS (hPTECs-3D), & hKidney. n = 9 for 2D CTL & hKidS & n = 3 for hPTECs-3D & hKidney. (j) Representative images of hKidS immunostained for p-GP, OCT2, & PTHR. All data are shown as the means ± s.e.m. *p < 0.05 compared with 2D or 3D control by unpaired Student’s t-test. Scale bar, 20 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31772214), licensed under a CC-BY license. Not internally tested by Novus Biologicals.