PIEZO1 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-10504
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Rabbit
Cited:
Mouse, Rat
Applications
Validated:
ELISA, Immunohistochemistry, Knockdown Validated, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Synthetic peptide derived from C-terminal domain of human Protein FAM38A.
Specificity
Reacts with human 287 kDa Protein FAM38A. Cross-reacts with mouse, rat, rabbit and other species due to sequence homology. Piezo-type mechanosensitive ion channel component 1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PIEZO1 Antibody - Azide and BSA Free
Western Blot: PIEZO1 Antibody [NBP2-10504] -
Western Blot: PIEZO1 Antibody [NBP2-10504] - PC2, TRPV4, & PIEZO1 localize to MLO-Y4 primary cilia & plasma membrane. (A–C) Localization of PC2, TRPV4, & PIEZO1 on MLO-Y4 primary cilia & plasma membrane. Acetylated alpha-tubulin is in enriched primary cilia. Scale bars, 10 μm. (D) Chemiluminescence-detected Western blot of PC2, TRPV4, PIEZO1, & actin protein in MLO-Y4s treated with scrambled control siRNA & Pkd2, Trpv4, or Piezo1 siRNA. (E) Quantified knockdown of Pkd2 (n = 4), Trpv4 (n = 10), & Piezo1 (n = 4) mRNA expression in MLO-Y4 cells treated with siRNA relative to controls (n = 10). Error bars show ±SEM. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26029358), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for PIEZO1 Antibody - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50-1:500
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Lyophilized powder, reconstitute to 1 mg/ml in distilled water.
Formulation
0.1M Tris , 0.1M Glycine and 2% Sucrose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PIEZO1
Long Name
Piezo-type mechanosensitive ion channel component 1
Alternate Names
FAM38A, KIAA0233, Mib, Protein FAM38A
Gene Symbol
PIEZO1
UniProt
Additional PIEZO1 Products
Product Documents for PIEZO1 Antibody - Azide and BSA Free
Product Specific Notices for PIEZO1 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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