PCP4 Antibody (CL5306)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-52479
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP3-52479]](https://resources.bio-techne.com/images/products/nbp3-52479_-immunohistochemistry-paraffin-639185435303404481.jpg "Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP3-52479]")
![Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP3-52479]](https://resources.bio-techne.com/images/products/nbp3-52479_-immunohistochemistry-paraffin-639185435300940508.jpg "Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP3-52479]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # CL5306
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from P48539, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for PCP4 Antibody (CL5306)
Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP3-52479]
Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.![Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP3-52479]](https://resources.bio-techne.com/images/products/nbp3-52479_-immunohistochemistry-paraffin-639185435302144251.jpg "Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP3-52479]")
Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP3-52479]
Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.![Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP3-52479]](https://resources.bio-techne.com/images/products/nbp3-52479_-immunohistochemistry-paraffin-639185435301922349.jpg "Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP3-52479]")
Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP3-52479]
Staining of human testis shows no positivity in cells in seminiferous ducts as expected.![Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP3-52479]](https://resources.bio-techne.com/images/products/nbp3-52479_-immunohistochemistry-paraffin-639185435304034043.jpg "Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP3-52479]")
![Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP3-52479]](https://resources.bio-techne.com/images/products/nbp3-52479_-immunohistochemistry-paraffin-639185435304420126.jpg "Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP3-52479]")
Applications for PCP4 Antibody (CL5306)
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Preservative
0.02% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PCP4
Alternate Names
brain specific polypeptide PEP19, 10Brain-specific antigen PCP-4, Brain-specific polypeptide PEP-19, PEP19, PEP-19, Purkinje cell protein 4
Gene Symbol
PCP4
Additional PCP4 Products
Product Specific Notices for PCP4 Antibody (CL5306)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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