What PAX6 antibody is recommend to use in ICC staining of human stem cells differentiated neural precursors?

Name: Pax6 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP2-19711

By performing a search on PAX6 then refining this to antibodies which have been validated with human samples and for ICC, I can see that we have three products to meet your requirements. You can see all three of these antibodies. These products are listed based on sales and citations, however all of our antibodies are covered by our 100% guarantee to work in the species and applications listed on our website and on our product datasheets. You can read more about our quality guarantee here.

Pax6 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19711

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Citations (4)

Product Datasheet / COA

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Bovine (100%), Canine (100%), Chicken (98%), Porcine (100%), Rabbit (100%), Rhesus Macaque (100%), Xenopus (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Pax6 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Pax6. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (94%).

Localization

Nucleus

Marker

Neural Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Pax6 Antibody - BSA Free

Immunohistochemistry-Frozen: Pax6 Antibody [NBP2-19711]

Immunohistochemistry-Frozen: Pax6 Antibody [NBP2-19711] - Frozen sectioned E13.5 Rat brain. Green: PAX6 protein stained by PAX6 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.

Immunoprecipitation: Pax6 Antibody [NBP2-19711]

Immunoprecipitation: PAX6 Antibody [NBP2-19711] - Sample: 1000 ug 293T whole cell lysate/extract A. 50 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of PAX6 protein by 2 ug of PAX6 antibody 10% SDS-PAGE The immunoprecipitated PAX6 protein was detected by PAX6 antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.

Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] -

Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse E10.5 embryo.
Green: Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI .
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] -

Immunocytochemistry/ Immunofluorescence: Pax6 Antibody [NBP2-19711] -

Immunocytochemistry/ Immunofluorescence: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI .

Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] -

Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat eye.
Green: Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI .
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] -

PAX6 antibody detects PAX6 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse E10.5 embryo.
Green: PAX6 stained by PAX6 antibody (NBP2-19711) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.
Blue: Hoechst 33342 staining.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Frozen: Pax6 Antibody [NBP2-19711] -

PAX6 antibody detects PAX6 protein at nucleus in embryonic mouse brain by immunohistochemical analysis.
Sample: Frozen section of embryonic mouse brain (mE12.5).
PAX6 antibody (NBP2-19711) diluted at 1:250.

Immunohistochemistry: Pax6 Antibody [NBP2-19711] -

PAX6 antibody detects PAX6 protein by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: PAX6 stained by PAX6 antibody (NBP2-19711) diluted at 1:200.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70C

Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] -

PAX6 antibody detects PAX6 protein by immunohistochemical analysis.
Samples: Paraffin-Embedded mouse retina.
Green: PAX6 protein stained by PAX6 antibody (NBP2-19711) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.
Blue: Fluoroshield with DAPI .
br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/ Immunofluorescence: Pax6 Antibody [NBP2-19711] -

PAX6 antibody detects PAX6 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PAX6 protein stained by PAX6 antibody (NBP2-19711) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:50.
Scale bar = 10 um.

Western Blot: Pax6 Antibody [NBP2-19711] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (NBP2-19711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Western Blot: Pax6 Antibody [NBP2-19711] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (NBP2-19711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Pax6 Antibody [NBP2-19711] -

Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (NBP2-19711) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Pax6 Antibody [NBP2-19711] -

Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (NBP2-19711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

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Applications for Pax6 Antibody - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:1000-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Pax6

This gene encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. Alternatively spliced transcript variants encoding either the same or different isoform have been found for this gene. [provided by RefSeq]

Long Name

Paired Box Gene 6

Alternate Names

keratitis), MGC17209, Oculorhombin, paired box 6, paired box protein Pax-6

Gene Symbol

PAX6

UniProt

P26367

Additional Pax6 Products

Product Documents for Pax6 Antibody - BSA Free

Product Datasheets

Download Product Datasheets

COA

Product Specific Notices for Pax6 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Pax6 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19711

Key Product Details

Species Reactivity

Validated:

Cited:

Predicted:

Applications

Validated:

Cited:

Label

Antibody Source

Format

Concentration

View all Pax6 Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Localization

Marker

Clonality

Host

Isotype

Theoretical MW

Scientific Data Images for Pax6 Antibody - BSA Free

Immunohistochemistry-Frozen: Pax6 Antibody [NBP2-19711]

Immunoprecipitation: Pax6 Antibody [NBP2-19711]

Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] -

Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] -

Immunocytochemistry/ Immunofluorescence: Pax6 Antibody [NBP2-19711] -

Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] -

Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] -

Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] -

Immunohistochemistry-Frozen: Pax6 Antibody [NBP2-19711] -

Immunohistochemistry: Pax6 Antibody [NBP2-19711] -

Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] -

Immunocytochemistry/ Immunofluorescence: Pax6 Antibody [NBP2-19711] -

Western Blot: Pax6 Antibody [NBP2-19711] -

Western Blot: Pax6 Antibody [NBP2-19711] -

Western Blot: Pax6 Antibody [NBP2-19711] -

Western Blot: Pax6 Antibody [NBP2-19711] -

Applications for Pax6 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Frozen

Immunohistochemistry-Paraffin

Immunoprecipitation

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: Pax6

Long Name

Alternate Names

Gene Symbol

UniProt

Additional Pax6 Products

Product Documents for Pax6 Antibody - BSA Free

Product Datasheets

COA

Certificate of Analysis

Product Specific Notices for Pax6 Antibody - BSA Free