non-muscle heavy chain 10 Myosin Antibody (GT246) - Azide and BSA Free

Name: non-muscle heavy chain 10 Myosin Antibody (GT246) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP3-13591

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13591

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # GT246

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human non-muscle heavy chain 10 Myosin. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Theoretical MW

229 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images

Western Blot: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591]

Western Blot: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle heavy chain 10 Myosin antibody [GT246] (NBP3-13591) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.

Western Blot: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591]

Western Blot: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle heavy chain 10 Myosin antibody [GT246] (NBP3-13591) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.

Western Blot: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591]

Western Blot: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle heavy chain 10 Myosin antibody [GT246] (NBP3-13591) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.

Western Blot: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591]

Immunohistochemistry-Paraffin: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591]

Immunohistochemistry-Paraffin: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591] - non-muscle heavy chain 10 Myosin antibody [GT246] detects non-muscle heavy chain 10 Myosin protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. non-muscle heavy chain 10 Myosin antibody [GT246] (NBP3-13591) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Frozen: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591] -

MYH10 antibody [GT246] detects MYH10 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: MYH10 stained by MYH10 antibody [GT246] (NBP3-13591) diluted at 1:250.
Blue: Fluoroshield with DAPI .

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Immunocytochemistry/ Immunofluorescence: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591] -

MYH10 antibody [GT246] detects MYH10 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MYH10 protein stained by MYH10 antibody [GT246] (NBP3-13591) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

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