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NEK2 Antibody (HL2511)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48693

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-48693

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2511

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein of human NEK2

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit NEK2 Antibody (HL2511) (NBP3-48693) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for NEK2 Antibody (HL2511)

NEK2 Antibody (HL2511)

Immunohistochemistry-Paraffin: NEK2 Antibody (HL2511) [NBP3-48693] -

NEK2 antibody [HL2511] detects NEK2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human testis.NEK2 stained by NEK2 antibody [HL2511] (NBP3-48693) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
NEK2 Antibody (HL2511)

Western Blot: NEK2 Antibody (HL2511) [NBP3-48693] -

Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NEK2 antibody [HL2511] (NBP3-48693) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
NEK2 Antibody (HL2511)

Western Blot: NEK2 Antibody (HL2511) [NBP3-48693] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NEK2 antibody [HL2511] (NBP3-48693) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for NEK2 Antibody (HL2511)

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NEK2

Protein kinase that is involved in mitotic regulation. May have a role at the G2-M transition. May also play a role in meiosis. Isoform 1 but not isoform 2 appears to play a role in centrosome splitting. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not

Long Name

NIMA [Never In Mitosis Gene A]-related Kinase 2

Alternate Names

HsPK21, NLK1

Gene Symbol

NEK2

Additional NEK2 Products

Product Documents for NEK2 Antibody (HL2511)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NEK2 Antibody (HL2511)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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