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Key Product Details

Species Reactivity

Mouse

Applications

Immunocytochemistry, Western Blot

Label

Alexa Fluor 750 (Excitation = 749 nm, Emission = 775 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 831629

Product Specifications

Immunogen

E. coli-derived recombinant mouse TGN38
His320-Leu349
Accession # Q62313

Specificity

Detects mouse TGN38 in ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for Mouse TGN38 Alexa Fluor® 750-conjugated Antibody

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TGN38

TGN38A (also Tgoln1, Trans-Golgi network integral membrane protein 1) is an integral membrane protein associated with intracellular protein trafficking. Although its predicted MW is 38 kDa, due to a high proline content and extensive polysialylation, it runs anomalously at 80-90 kDa in SDS-PAGE. Tgoln1 is ubiquitously expressed and is generally associated with the trans-Golgi complex, a structure adjacent to the trans-component of the Golgi Apparatus. In this complex, both secretory and membrane proteins are sorted and forwarded to various compartments such as lysosomes, endosomes, the cell membrane and secretory granules. TGN38A apparently cycles between the trans-Golgi network and the cell membrane, returning to the trans-Golgi via the endosomal system. Part of its mobility is mediated by an interaction between TGN38A, neurabin and actin. Integrin beta1 is one molecule suggested to be transported by TGN38A.  Mature mouse TGN38A is a 336 amino acid (aa) type I transmembrane glycoprotein.  It contains a 281 aa extracellular region (aa 18-298) plus a 34 aa cytoplasmic domain.  The extracellular region possesses six sequential octapeptide repeats (aa 131-178) plus two utilized phosphorylation sites (Ser230 and Ser231).  The cytoplasmic domain contains an endocytosis signal (Ser344-Leu349) that requires a free hydroxyl on Ser344 for proper routing. The mouse genome has two Tgn38 genes, Tgn38A and Tgn38B/Tgoln2.  They differ in two ways. First there is a two aa insertion after Pro47, and an eight aa insertion after Thr154 in Tgn38B and, second, Tgn38B expression is restricted, occurring in mouse strain ICR, while Tgn38A is widespread and found in strains ICR, BALB/c and C57BL/6.  Over aa 320-349, mouse and rat Tgoln1 are identical in aa sequence.  There is no strict human structural counterpart to mouse Tgoln1/Tgn38A.  Human does, however, possess a similar functioning molecule termed TGN46/48/51, and over the short aa stretch 320-349, mouse and human TGN molecules share 96% aa sequence identity.

Long Name

Trans-golgi Network Protein

Alternate Names

TGN46, TGN51, Tgoln1, Tgoln2, Ttgn1

Entrez Gene IDs

10618 (Human); 22134 (Mouse); 192152 (Rat)

Gene Symbol

TGOLN2

UniProt

Additional TGN38 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse TGN38 Alexa Fluor® 750-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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