Mouse Semaphorin 4D/CD100 Alexa Fluor™ Plus 555-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB52351AFP555
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry, CyTOF-ready
Label
Alexa Fluor Plus 555 (Excitation = 558 nm, Emission = 572 nm)
Antibody Source
Monoclonal Rat IgG2B Clone # 547412
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4D/CD100
Specificity
Detects mouse Semaphorin 4D/CD100 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Semaphorin 3A, 3B, 3C, 3D, 3E, 3F, 4A, 4B, 4C, 4D, 4G, 5A, 5B, 6A, 6B, 6C, 6D, or 7A is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Applications
Application
Recommended Usage
CyTOF-ready
Optimal dilution of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Semaphorin 4D/CD100
Semaphorin 4D (Sema4D/CD100, previously sem J, G or C-like 2) is a widely expressed 150 kDa type I transmembrane glycoprotein of the Class 4 Semaphorin family with activity in the immune and nervous systems. The 861 amino acid (aa) Sema4D contains a 710 aa extracellular domain (ECD) with a sema domain and an immunoglobulin-like domain. Within the ECD region used as an immunogen, mouse Sema4D shares 87% and 93% aa identity with human and rat Sema4D, respectively. A soluble, active 120 kDa form is produced proteolytically upon activation of B and T cells. Sema4D binds Plexin-B1, but also CD72 on B and dendritic cells. In developing neurons, Sema-4D assists in guidance. Sema4D is proangiogenic when expressed by tumor-associated macrophages.
Alternate Names
A8, BB18, CD100, COLL-4, M-sema-G, SEMA4D, SEMAJ
Gene Symbol
SEMA4D
UniProt
Additional Semaphorin 4D/CD100 Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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