Detects mouse and rat Transglutaminase 2/TGM2 in Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant human (rh) TGM2 and rhTGM7 is observed, and less than 1% cross‑reactivity with rhTGM3 and rhTGM4 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Mouse/Rat Transglutaminase 2/TGM2 Biotinylated Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Transglutaminase 2/TGM2 (Catalog # 5418-TG)

Formulation, Preparation, and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Transglutaminase 2/TGM2

Transglutaminase 2 (TG2), encoded by the Tgm2 gene, is also known as tissue Transglutaminase (tTG), Transglutaminase C (TGC), and protein-glutamine-gamma -glutamyltransferase. It belongs to the family of transglutaminases that catalyze the posttranslational modification of proteins via calcium dependent cross-linking reactions (1-3). In addition to its function in protein cross-linking, TGM2 is also capable of hydrolyzing both GTP and ATP (4) and has intrinsic kinase activity (5). TGM2 has been implicated in a variety of human diseases including celiac disease, inclusion body myositis, atherosclerosis, and neurodegenerative diseases (6-7).

References

Gentile, V. et al. (1991) J. Biol. Chem. 266:478.
Chen, J.S.K. and Mehta K. (1999) Internat. J. Biochem. Cell Biol. 31:817.
Griffin, M. et al. (2002) Biochem. J. 368:377.
Lai, T.S. et al. (1998) J. Biol. Chem. 273:1776.
Mishra, S. et al. (2007) J. Biol. Chem. 282:18108.
Kim, S-Y. et al. (2002) Neurochem. Int. 40:85.
Lesort, M. et al. (2000) Prog. Neurobiol. 61:439.

Alternate Names

TGC, TGM2, Transglutaminase 2, tTG

Entrez Gene IDs

7052 (Human); 21817 (Mouse); 56083 (Rat)

Gene Symbol

TGM2

UniProt

P21981

Additional Transglutaminase 2/TGM2 Products

Product Documents for Mouse/Rat Transglutaminase 2/TGM2 Biotinylated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse/Rat Transglutaminase 2/TGM2 Biotinylated Antibody

For research use only

Citations
Reviews

Mouse/Rat Transglutaminase 2/TGM2 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF5418

Key Product Details

Species Reactivity

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Product Specifications

Immunogen

Specificity