Mouse/Rat Carboxylesterase 3/CES3 Alexa Fluor™ Plus 555-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5985AFP555
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Western Blot, Immunoprecipitation
Label
Alexa Fluor Plus 555 (Excitation = 558 nm, Emission = 572 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Carboxylesterase 3/CES3
Specificity
Detects recombinant mouse Carboxylesterase 3/CES3 in direct ELISAs and Western blots. Detects intrinsic CES3 in mouse and rat tissues in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CES1, rhCES2, recombinant mouse (rm) CES2, and rmCES5 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications
Application
Recommended Usage
Immunoprecipitation
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Carboxylesterase 3/CES3
Carboxylesterase 3 is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds (1, 2). These carboxylesterases are widely distributed in mammalian tissues and have broad substrate specificity ranging from small molecule esters such as phenylester to long-chain fatty acid esters and thioesters. Because many ester-containing drugs require the ester linkage to improve the bioavailability of therapeutic agents, CESs play a major role in drug metabolism and activation. CES3 is also known as triacylglycerol hydrolase for its lipolytic activity. It has been reported to be the major lipase in white adipose tissue (3). Mouse CES3 shares 93% homology with rat CES3.
References
- Redinbo, M. R. and Potter, P.M. (2005) Drug Discovery Today. 10:313.
- Satoh, T. and Hosokawa, M. (2006) Chem.-Biol. Interactions. 162:195.
- Soni, K. G. et al. (2004) J. Biol. Chem. 279:40683.
Alternate Names
CES3
Gene Symbol
CES3
UniProt
Additional Carboxylesterase 3/CES3 Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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