Mouse Mast Cell Protease-1/Mcpt1 Alexa Fluor™ Plus 647-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB5146AFP647
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Alexa Fluor Plus 647 (Excitation = 658 nm, Emission = 675 nm)
Antibody Source
Monoclonal Rat IgG2B Clone # 285008
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Mast Cell Protease‑1/Mcpt1
Specificity
Detects mouse Mast Cell Protease‑1/Mcpt1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) MCP-6, -7, or ‑11 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Mast Cell Protease-1/Mcpt1
Mast cell protease-1 (MCPT-1), also known as beta-chymase, is a member of the Chymase family of chymotrypsin-like serine proteases (1). mMCPT-1 is a mast cell protease predominantly expressed in intestinal mucosal mast cells where it promotes mucosal permeability in intestinal allergic hypersensitivity reactions (2). Its activation is completed by the removal of a two residue N-terminal propeptide by a dipeptidyl peptidase (Cathepsin C) (3). Like human alpha-Chymase, MCPT-1 is capable of the conversion of angiotensin I to angiotensin II, which plays a key role in the regulation of arterial pressure (4). Studies have shown that specific chymase inhibitors are able to diminish the development of abdominal aortic aneurysm and reduce the adhesion formation after cardiac surgery in hamsters (5, 6). Therefore, the development of specific inhibitors of chymase activity has been a pharmacologic strategy to develop therapeutic agents.
References
- Caughey, G.H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.), p. 1531 Academic Press, San Diego.
- Wastling, J.M. et al. (1998) Am. J. Pathol. 153:491.
- Murakami, M. et al. (1995) J. Biol. Chem. 270:2218.
- Saito, K. et al. (2003) Biochem. Biophys. Res. Commun. 302:773.
- Tsunemi, K. et al. (2004) J. Pharmacol. Exp. Ther. 309:879.
- Soga, Y. et al. (2004) J. Thorac. Cardiovasc. Surg. 127:72.
Alternate Names
Mast Cell Protease1, Mcp1, MMPC-1
Gene Symbol
MCPT1
UniProt
Additional Mast Cell Protease-1/Mcpt1 Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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