Mouse HSF1 Alexa Fluor™ Plus 594-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB7130AFP594

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FAB7130AFP594-100UG
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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry

Label

Alexa Fluor Plus 594 (Excitation = 590 nm, Emission = 618 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 744509

View all HSF1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse HSF1

Specificity

Detects mouse HSF1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human HSF2 or recombinant mouse HSF4 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse HSF1 Alexa Fluor™ Plus 594-conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: HSF1

Heat Shock Factor 1 (HSF1) is a 75-80 kDa constitutively expressed protein that functions as a transcriptional regulator under heat shock conditions. Heat stress induces the serine hyperphosphorylation, sumoylation, trimerization, and translocation of HSF1 to the nucleus. Nuclear HSF1 binds to heat shock elements (HSE) and promotes the transcription of genes important in cell protection from heat stress. Mouse HSF1 contains a DNA binding domain (aa 16-120), two hydrophobic oligomerization motifs (aa 137-212 and aa 378-407), a regulatory domain (aa 221-310), and a transactivation domain (aa 367-525). Alternate splicing of mouse HSF1 generates a short isoform that lacks 22 amino acids of the transactivation domain. Within aa 124-247, mouse HSF1 shares 94% and 99% aa sequence identity with human and rat HSF1, respectively.

Long Name

Heat Shock Transcription Factor 1

Alternate Names

HSTF1

Entrez Gene IDs

3297 (Human); 154991 (Mouse); 79245 (Rat)

Gene Symbol

HSF1

UniProt

P38532 (Mouse)

Additional HSF1 Products

Product Documents

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse HSF1 Alexa Fluor™ Plus 594-conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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