3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Sheep Anti-Mouse FPRP/PTGFRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4495, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Northern-Lights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).

Applications for Mouse FPRP/PTGFRN Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: 3T3-L1 mouse embryonic fibroblast adipose-like cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse FPRP/PTGFRN

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FPRP/PTGFRN

FPRP (F_{2 alpha} Prostaglandin receptor Regulatory Protein; also CD315, EWI-F and CD9 partner-1/P-1) is a 130 kDa type I transmembrane (TM) glycoprotein member of the EWI subfamily, Ig superfamily of molecules. It associates with the 7-TM prostaglandin F_{2 alpha} receptor, inhibiting its activity. It also forms complexes with 4-TM CD9 and CD81, linking these molecules to the intracellular actin cytoskeleton. Mature mouse FPRP is 858 amino acids (aa) in length. Its extracellular domain (ECD) contains six C2-type Ig-like domains (aa 22‑813) plus a Pro-Thr-Glu-Leu ER retention signal. The ECD of mouse FPRP (aa 22‑832) shares 89% and 98% aa identity with the ECD of human and rat FPRP, respectively.

Long Name

Prostaglandin F2-alpha Receptor Regulatory Protein

Alternate Names

CD315, CD9P-1, PTGFRN

Entrez Gene IDs

5738 (Human); 19221 (Mouse); 29602 (Rat)

Gene Symbol

PTGFRN

UniProt

NP_035327

Additional FPRP/PTGFRN Products

Product Documents for Mouse FPRP/PTGFRN Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse FPRP/PTGFRN Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse FPRP/PTGFRN Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4495

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all FPRP/PTGFRN Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse FPRP/PTGFRN Antibody

Detection of FPRP/PTGFRN in 3T3‑L1 Mouse Cell Line by Flow Cytometry.