Mouse F4/80 Antibody

Name: Mouse F4/80 Antibody
Brand: R&D Systems
SKU: MAB11811

R&D Systems, part of Bio-Techne | Catalog # MAB11811

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot, COMET

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG_2A Clone # 1123826

View all F4/80 Antibodies »

Product Specifications

Immunogen

Chinese Hamster Ovary cell line, CHO-derived mouse EMR-1
Met1-Ser590
Accession # Q61549

Specificity

Detects recombinant mouse F4/80 protein in Direct ELISA.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Scientific Data Images for Mouse F4/80 Antibody

Detection of EMR1 in Mouse Spleen via seqIF™ staining on COMET™

EMR-1 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Rat Anti-Mouse EMR-1 Monoclonal Antibody (Catalog # MAB11811) at 3ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of EMR1 in Mouse Testis via seqIF™staining on COMET™

EMR-1 was detected in immersion fixed paraffin-embedded sections of mouse testis using Rat Anti-Mouse EMR-1 Monoclonal Antibody (Catalog # MAB11811) at 3ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of EMR1 in Mouse Liver via seqIF™staining on COMET™

EMR-1 was detected in immersion fixed paraffin-embedded sections of mouse liver using Rat Anti-Mouse EMR-1 Monoclonal Antibody (Catalog # MAB11811) at 3ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of EMR1 in Mouse Kidney viaseqIF™ staining on COMET™

EMR-1 was detected in immersion fixed paraffin-embedded sections of mouse kidney using Rat Anti-Mouse EMR-1 Monoclonal Antibody (Catalog # MAB11811) at 3ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of Mouse F4/80 by Western Blot.

Western Blot shows lysates of mouse spleen and J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 2 µg/ml of Rat Anti-Mouse F4/80 Monoclonal Antibody (Catalog # MAB11811) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for F4/80 at approximately 130-300 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Perfusion fixed paraffin-embedded sections of mouse liver

F4/80 was detected in perfusion fixed paraffin-embedded sections of mouse liver using Rat Anti-Mouse F4/80 Monoclonal Antibody (Catalog # MAB11811) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Perfusion fixed paraffin-embedded sections of mouse spleen

F4/80 was detected in perfusion fixed paraffin-embedded sections of mouse spleen using Rat Anti-Mouse F4/80 Monoclonal Antibody (Catalog # MAB11811) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Applications for Mouse F4/80 Antibody

Application

Recommended Usage

COMET

3 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse spleen, mouse testis, mouse liver and mouse kidney.

Immunohistochemistry

0.25-25 µg/mL
Sample: Perfusion fixed paraffin-embedded sections of mouse spleen and sections of mouse liver

Western Blot

2 µg/mL
Sample: Mouse spleen and J774A.1 mouse reticulum cell sarcoma macrophage cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: F4/80

F4/80, also known as EMR1, is a 160 kDa extensively glycosylated protein in the EGF-TM7 family of adhesion molecules. It is primarily expressed on macrophages and dendritic cells in mouse but is restricted to eosinophils in human. F4/80 plays a role in the development of peripheral tolerance.

Alternate Names

DD7A5-7, Gpf480, Ly71

Entrez Gene IDs

13733 (Mouse); 316137 (Rat)

Gene Symbol

ADGRE1

UniProt

Q61549

Additional F4/80 Products

Product Documents for Mouse F4/80 Antibody

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Product Specific Notices for Mouse F4/80 Antibody

For research use only

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