Mouse ENPP-7/Alk-SMase Alexa Fluor™ Plus 594-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5160AFP594
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot, Immunoprecipitation
Label
Alexa Fluor Plus 594 (Excitation = 590 nm, Emission = 618 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ENPP‑7/Alk‑SMase
Specificity
Detects mouse ENPP‑7/Alk‑SMase in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant human ENPP-7 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: ENPP-7/Alk-SMase
ENPP7 (ectonucleotide pyrophosphatase/phosphodiesterase 7), also known as alkaline sphingomyelinase (Alk-SMase) is expressed in the intestines and in bile (1). It shares 30-36% homology with the members of the nucleotide pyrophosphatase/phosphodiesterese (NPP) family while sharing no similarities with neutral or acid SMase (2). Its main function is the digestion of dietary sphingomyelin by hydrolyzing sphingomyelin into ceramide and phosphorylcholine. ENPP7 is reported to hydrolyse and inactivate platelet-activating factor (PAF) by a phospholipase C-type activity (3). Studies show a decrease in ENPP7 activity in human colorectal adenocarcinomas and human colorectal carcinomas, which indicate a potential role of ENPP7 in human colon cancer (4, 5).
References
- Duan, R-D. et al. (2006) Biochim. Biophys. 1761:281.
- Duan, R-D. et al. (2003) J. Biol. Chem. 278:38528.
- Wu, J. et al. (2006) Biochem J. 394:299.
- Hertervig, E. et al. (1996) Cancer. 79:448.
- Hertervig, E. et al. (1999) Br. J. Cancer. 81:232.
Long Name
Ectonucleotide Pyrophosphatase/Phosphodiesterase 7
Alternate Names
Alk-SMase, ENPP7, NPP7
Gene Symbol
ENPP7
UniProt
Additional ENPP-7/Alk-SMase Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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