Mouse Amnionless Alexa Fluor® 405-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7139V
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Label
Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Amnionless
Ala20-Gly362
Accession # Q99JB7
Ala20-Gly362
Accession # Q99JB7
Specificity
Detects mouse Amnionless in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Amnionless is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Mouse Amnionless Alexa Fluor® 405-conjugated Antibody
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Amnionless
AMN (Amnionless) is a 45-49 kDa glycoprotein that is expressed by three cell types in mouse; extraembryonic visceral endoderm, proximal tubule renal epithelium, and absorption intestinal epithelium. AMN is required for gastrulation in rodent (but not human), and participates in Vitamin B12 absorption in the gut. In particular, AMN forms a membrane complex with Cubilin, promoting its proper orientation in the membrane and subsequent binding to the Intrinsic factor: VitB12 complex. Mature mouse AMN is a 439 amino acid (aa) type I transmembrane protein. It contains a 343 aa extracellular region (aa 20-362) that possesses one VWFC domain (aa 203‑254), and a 75 aa cytoplasmic tail. In human, AMN may undergo cleavage to generate a 35 kDa soluble fragment. Over aa 20-362, mouse AMN shares 92% and 67% aa sequence identity with rat and human AMN, respectively.
Additional Amnionless Products
Product Specific Notices for Mouse Amnionless Alexa Fluor® 405-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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