MNX1/HLXB9 Antibody (JE32-08)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32417
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE32-08 expressed in HEK293
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human MNX1/HLXB9 aa 276-325 / 401. (Uniprot: P50219)
Localization
Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit MNX1/HLXB9 Antibody (JE32-08) (NBP3-32417) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for MNX1/HLXB9 Antibody (JE32-08)
Western Blot: MNX1/HLXB9 Antibody (JE32-08) [NBP3-32417]
Western Blot: MNX1/HLXB9 Antibody (JE32-08) [NBP3-32417] - Western blot analysis of MNX1/HLXB9 on different lysates with Rabbit anti-MNX1/HLXB9 antibody (NBP3-32417) at 1/1,000 dilution.Lane 1: HeLa cell lysate (15 ug/Lane)
Lane 2: Raji cell lysate (15 ug/Lane)
Lane 3: MCF7 cell lysate (15 ug/Lane)
Lane 4: HepG2 cell lysate (15 ug/Lane)
Lane 5: Huh7 cell lysate (15 ug/Lane)
Lane 6: PANC-1 cell lysate (15 ug/Lane)
Lane 7: SW1990 cell lysate (15 ug/Lane)
Lane 8: Mouse pancreas tissue lysate (30 ug/Lane)
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 5 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32417) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Applications for MNX1/HLXB9 Antibody (JE32-08)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HB9
Long Name
Homeo Box HB9
Alternate Names
HLXB9, HOXHB9, MNR2, MNX1, SCRA1
Gene Symbol
MNX1
Additional HB9 Products
Product Documents for MNX1/HLXB9 Antibody (JE32-08)
Product Specific Notices for MNX1/HLXB9 Antibody (JE32-08)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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