MBD3 Antibody (9X1J7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16718

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16718-100ul
NBP3-16718-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9X1J7 expressed in HEK293

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MBD3 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MBD3 (O95983). MERKRWECPALPQGWEREEVPRRSGLSAGHRDVFYYSPSGKKFRSKPQLARYLGGSMDLSTFDFRTGKMLMSKMNKSRQRVRYDSSNQVKGKPDLNTALP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit MBD3 Antibody (9X1J7) (NBP3-16718) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for MBD3 Antibody (9X1J7)

Western Blot: MBD3 Antibody (9X1J7) [NBP3-16718]

Western Blot: MBD3 Antibody (9X1J7) [NBP3-16718]

Western Blot: MBD3 Antibody (9X1J7) [NBP3-16718] - Western blot analysis of extracts of various cell lines, using MBD3 Rabbit mAb (NBP3-16718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: MBD3 Antibody (9X1J7) [NBP3-16718]

Immunohistochemistry-Paraffin: MBD3 Antibody (9X1J7) [NBP3-16718]

Immunohistochemistry-Paraffin: MBD3 Antibody (9X1J7) [NBP3-16718] - Immunohistochemistry of paraffin-embedded human colon carcinoma using MBD3 Rabbit mAb (NBP3-16718) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western Blot: MBD3 Antibody (9X1J7) [NBP3-16718]

Western Blot: MBD3 Antibody (9X1J7) [NBP3-16718]

Western Blot: MBD3 Antibody (9X1J7) [NBP3-16718] - Western blot analysis of extracts of various cell lines, using MBD3 Rabbit mAb (NBP3-16718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Applications for MBD3 Antibody (9X1J7)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MBD3

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (4). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (3). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (2). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (1).

Long Name

Methyl-CpG Binding Domain Protein 3

Alternate Names

methyl-CpG binding domain protein 3, methyl-CpG-binding domain protein 3, Methyl-CpG-binding protein MBD3

Gene Symbol

MBD3

Additional MBD3 Products

Product Documents for MBD3 Antibody (9X1J7)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MBD3 Antibody (9X1J7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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