Maxi Potassium channel alpha Antibody (HL3164)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48775

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-48775
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL3164

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Maxi Potassium channel alpha/KCNMA1 Antibodies »

Product Specifications

Immunogen

The immunogen used to generate this antibody corresponds to human Maxi Potassium channel alpha.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Maxi Potassium channel alpha Antibody (HL3164) (NBP3-48775) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Maxi Potassium channel alpha Antibody (HL3164)

Maxi Potassium channel alpha Antibody (HL3164)

Western Blot: Maxi Potassium channel alpha Antibody (HL3164) [NBP3-48775] -

Boiled and unboiled rat tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Maxi Potassium channel alpha antibody [HL3164] (NBP3-48775) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Maxi Potassium channel alpha Antibody (HL3164)

Western Blot: Maxi Potassium channel alpha Antibody (HL3164) [NBP3-48775] -

Boiled and unboiled various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Maxi Potassium channel alpha antibody [HL3164] (NBP3-48775) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Maxi Potassium channel alpha Antibody (HL3164)

Immunocytochemistry/ Immunofluorescence: Maxi Potassium channel alpha Antibody (HL3164) [NBP3-48775] -

Maxi Potassium channel alpha antibody [HL3164] detects Maxi Potassium channel alpha protein by immunofluorescent analysis.Sample: DIV11 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Maxi Potassium channel alpha stained by Maxi Potassium channel alpha antibody [HL3164] (NBP3-48775) diluted at 1:250.Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.Blue: Fluoroshield with DAPI .
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Applications for Maxi Potassium channel alpha Antibody (HL3164)

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Maxi Potassium channel alpha/KCNMA1

Function: Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX). The protein was initially thought to contain two functionally distinct parts: The core channel (from the N-terminus to the S9 segment) that mediates the channel activity, and the cytoplasmic tail (from the S9 segment to the C-terminus) that mediates the calcium sensing. The situation is however more complex, since the core channel also contains binding sites for Ca(2+) and Mg(2+).; Defects in KCNMA1 are the cause of generalized epilepsy and paroxysmal dyskinesia (GEPD). Epilepsy is one of the most common and debilitating neurological disorders. Paroxysmal dyskinesias are neurological disorders characterized by sudden, unpredictable, disabling attacks of involuntary movement often requiring life-long treatment. The coexistence of epilepsy and paroxysmal dyskinesia in the same individual or family is an increasingly recognized phenomenon. Patients manifest absence seizures, generalized tonic-clonic seizures, paroxysmal nonkinesigenic dyskinesia, involuntary dystonic or choreiform movements. Onset is usually in childhood and patients may have seizures only, dyskinesia only, or both.; Enzyme regulation: Ethanol and carbon monoxide-bound heme increase channel activation. Heme inhibits channel activation.; Subcellular location: Membrane, Multi-pass membrane protein.; Tissue specificity: Widely expressed. Except in myocytes, it is almost ubiquitously expressed.

Long Name

Calcium-activated potassium channel subunit alpha-1

Alternate Names

BK channel, BKCA alpha, hSlo, K(VCA)alpha, KCa1.1, KCNMA, KCNMA1, Maxi K channel, MaxiK, SLO, Slo homolog, Slo-alpha, Slo1, Slowpoke homolog

Gene Symbol

KCNMA1

Additional Maxi Potassium channel alpha/KCNMA1 Products

Product Documents for Maxi Potassium channel alpha Antibody (HL3164)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Maxi Potassium channel alpha Antibody (HL3164)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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