KAT1 Antibody (23GB2090)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50997

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NBP3-50997-100ul
NBP3-50997-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB2090

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all KAT1 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human KAT1 / HAT1 (Uniprot: O14929)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KAT1 Antibody (23GB2090)

KAT1 Antibody (23GB2090)

Immunocytochemistry/ Immunofluorescence: KAT1 Antibody (23GB2090)[NBP3-50997] -

KAT1 antibody (1:1,000), Top panel: wild-type (WT); Bottom panal:Histone acetyltransferase 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Histone acetyltransferase 1 was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um.
KAT1 Antibody (23GB2090)

Immunocytochemistry/ Immunofluorescence: KAT1 Antibody (23GB2090)[NBP3-50997] -

Immunocytochemical staining of Hela cells with KAT1 antibody (1:1,000). Nuclei were stained blue with DAPI; KAT1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
KAT1 Antibody (23GB2090)

Flow Cytometry: KAT1 Antibody (23GB2090)[NBP3-50997] -

Flow cytometric analysis of KAT1expression in HeLa cells using KAT1 antibody (1:2,000). Green, isotype control; red, Histone acetyltransferase 1.
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Applications for KAT1 Antibody (23GB2090)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: KAT1

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBPassociated factor), p300/CBP, HAT1, and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6, have been identified as histone deacetylases.

Alternate Names

EC 2.3.1.48, histone acetyltransferase 1, histone acetyltransferase type B catalytic subunit, KAT1

Gene Symbol

HAT1

Additional KAT1 Products

Product Documents for KAT1 Antibody (23GB2090)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for KAT1 Antibody (23GB2090)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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