ISG15/UCRP Antibody (HL2017) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25537
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation
Species Reactivity
Human, Mouse, Rat, Feline
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2017 expressed in HEK293
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fragment of human ISG15/UCRP
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit ISG15/UCRP Antibody (HL2017) - Azide and BSA Free (NBP3-25537) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for ISG15/UCRP Antibody (HL2017) - Azide and BSA Free
Western Blot: ISG15/UCRP Antibody (HL2017) - Azide and BSA Free [NBP3-25537] -
Western Blot: ISG15/UCRP Antibody (HL2017) - Azide and BSA Free [NBP3-25537] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody [HL2017] (NBP3-25537) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunocytochemistry/Immunofluorescence: ISG15/UCRP Antibody (HL2017) - Azide and BSA Free [NBP3-25537] -
Immunocytochemistry/Immunofluorescence: ISG15/UCRP Antibody (HL2017) - Azide and BSA Free [NBP3-25537] - ISG15 antibody [HL2017] detects ISG15 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: ISG15 stained by ISG15 antibody [HL2017] (NBP3-25537) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10um.
Western Blot: ISG15/UCRP Antibody (HL2017) - Azide and BSA Free [NBP3-25537] -
Western Blot: ISG15/UCRP Antibody (HL2017) - Azide and BSA Free [NBP3-25537] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody [HL2017] (NBP3-25537) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Applications for ISG15/UCRP Antibody (HL2017) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ISG15/UCRP
Long Name
ISG15 Ubiquitin-like Modifier
Alternate Names
G1P2, IFI15, IP17, UCRP
Gene Symbol
ISG15
Additional ISG15/UCRP Products
Product Documents for ISG15/UCRP Antibody (HL2017) - Azide and BSA Free
Product Specific Notices for ISG15/UCRP Antibody (HL2017) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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