IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16786
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4X3S10 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human IMP Dehydrogenase 2/IMPDH2 (P12268). QANEVRKVKKYEQGFITDPVVLSPKDRVRDVFEAKARHGFCGIPITDTGRMGSRLVGIISSRDIDFLKEEEHDCFLEEIMTKREDLVVAPAGITLKEANEILQRSKKGKLPIVNEDDELVAIIARTDLKKNRDYPLASKDAKKQLLCGAAIGTHEDDKYRLDLLAQAGVDVVVLDSSQGNSIFQINMIKYIKDKYPNLQVI
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10) (NBP3-16786) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10)
Western Blot: IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10) [NBP3-16786] -
Western Blot: IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10) [NBP3-16786] - Western blot analysis of lysates from wild type(WT) and IMP Dehydrogenase 2/IMPDH2 knockout (KO) NIH/3T3(KO) cells, using [KO Validated] IMP Dehydrogenase 2/IMPDH2 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 3s.
Immunocytochemistry/ Immunofluorescence: IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10) [NBP3-16786] -
Immunocytochemistry/ Immunofluorescence: IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10) [NBP3-16786] - Confocal imaging of C6 cells using [KO Validated] IMP Dehydrogenase 2/IMPDH2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Immunocytochemistry/ Immunofluorescence: IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10) [NBP3-16786] -
Immunocytochemistry/ Immunofluorescence: IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10) [NBP3-16786] - Confocal imaging of NIH/3T3 cells using [KO Validated] IMP Dehydrogenase 2/IMPDH2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.Applications for IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IMP Dehydrogenase 2/IMPDH2
Long Name
Inosine 5'-Monophosphate Dehydrogenase 2
Alternate Names
IMDH2, IMP Oxireductase 2, IMPD2, IMPDH2
Gene Symbol
IMPDH2
Additional IMP Dehydrogenase 2/IMPDH2 Products
Product Documents for IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10)
Product Specific Notices for IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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