HYPE Antibody (3F5) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # H00011153-M02
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3F5
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
HYPE (AAH01342, 1 a.a. ~ 449 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAVTEPKWVSVWSRFLWVTLLSMVLGSLLALLLPLGAVEEQCLAVLKGLYLLRSKPDRAQHAATKCTSPSTELSITSRGATLLVAKTKASPAGKLEARAALNQALEMKRQGKREKAQKLFMHALKMDPDFVDALTEFGIFSEEDKDIIQADYLYTRALTISPYHEKALVNRDRTLPLVEEIDQRYFSIIDSKVKKVMSIPKGNSALRRVMEETYYHHIYHTVAIEGNTLTLSEIRHILETRYAVPGKSLEEQNEVIGMHAAMKYINTTLVSRIGSVTISDVLEIHRRVLGYVDPVEAGRFRTTQVLVGHHIPPHPQDVEKQMQEFVQWLNSEEAMNLHPVEFAALAHYKLVYIHPFIDGNGRTSRLLMNLILMQAGYPPITIRKEQRSDYYHVLEAANEGDVRPFIRFIAKCTETTLDTLLFATTEYSVALPEAQPNHSGFKETLPVKP
Specificity
HYPE - Huntingtin interacting protein E (3F5)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Novus Biologicals Mouse HYPE Antibody (3F5) - Azide and BSA Free (H00011153-M02) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for HYPE Antibody (3F5) - Azide and BSA Free
![Western Blot: HYPE Antibody (3F5) [H00011153-M02]](https://resources.bio-techne.com/images/products/HYPE-Antibody-3F5-Western-Blot-H00011153-M02-img0002.jpg "Western Blot: HYPE Antibody (3F5) [H00011153-M02]")
Western Blot: HYPE Antibody (3F5) [H00011153-M02]
Western Blot: HYPE Antibody (3F5) [H00011153-M02] - Analysis of FICD expression in transfected 293T cell line by HYPE monoclonal antibody (M02), clone 3F5.Lane 1: FICD transfected lysate (Predicted MW: 51.8 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: HYPE Antibody (3F5) [H00011153-M02] - Detection limit for recombinant GST tagged FICD is 1 ng/ml as a capture antibody.
Applications for HYPE Antibody (3F5) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: HYPE
Alternate Names
AMPylator FICD, EC 2.7.7.n1, FIC domain containing, FIC domain-containing protein, fic S-phase protein cell division homolog, HIP-13, HIP13UNQ3041, huntingtin interacting protein 13, Huntingtin interacting protein E, huntingtin interactor protein E, Huntingtin yeast partner E, Huntingtin-interacting protein 13, Huntingtin-interacting protein E, HYPEadenosine monophosphate-protein transferase FICD, MGC5623
Entrez Gene IDs
11153 (Human)
Gene Symbol
FICD
UniProt
Additional HYPE Products
Product Documents for HYPE Antibody (3F5) - Azide and BSA Free
Product Specific Notices for HYPE Antibody (3F5) - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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