Human TIN-Ag Alexa Fluor® 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6797G
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human TIN-Ag
Glu20-Pro476
Accession # Q6NSC1
Glu20-Pro476
Accession # Q6NSC1
Specificity
Detects human TIN-Ag in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human TINAGL1 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human TIN-Ag Alexa Fluor® 488-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: TIN-Ag
TIN-Ag (Tubulointerstitial nephritis antigen) is a 48-58 kDa glycoprotein member of the peptidase C1 family of molecules. It is secreted by renal tubule epithelium and (presumably) small intestine columnar epithelium. TIN-Ag is an integral component of the renal tubule basement membrane (BM), and appears to promote proper BM matrix organization, block laminin polymerization, and serve as a receptor for the epithelial integrins alpha3 beta1 and alphav beta3. Epithelial dissociation from the BM is associated with an epithelial-to-mesenchymal transition. Human TIN-Ag is synthesized as a 476 amino acid (aa) preproprecursor. It contains a 19 aa signal sequence, a 30 aa furin-cleavable prosegment (aa 20-49), and a 427 aa mature region (aa 50-476). Within the mature region is an SMB domain (aa 61-106), one vWFC domain (aa 119‑154), and a nonenzymatic peptidase C1A region (aa 218-466). Multiple splice variants are possible, and potential isoforms may show an alternative start site at Met322, or a combined deletion of aa 119‑169 plus 209-300, or a combination of an alternative start site at Met19 coupled to a 13 aa substitution for aa 209-476. Over aa 20-476, human proTIN-Ag shares 86% aa identity with mouse proTIN-Ag; over the mature region (aa 50-476), human TIN-Ag shares 89% aa identity with mouse TIN-Ag.
Long Name
Tubulointerstitial Nephritis Antigen
Alternate Names
TIN1, TIN2, TINAg
Gene Symbol
TINAG
UniProt
Additional TIN-Ag Products
Product Specific Notices for Human TIN-Ag Alexa Fluor® 488-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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