Detects human SALM4/LRFN3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human SALM3 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human SALM4/LRFN3 Alexa Fluor® 647-conjugated Antibody

Application

Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: SALM4/LRFN3

Synaptic adhesion-like molecule 4 (SALM4; also leucine-rich repeat and fibronectin type-III domain-containing protein 3 (Lrfn3) is an approximately 90 kDa member of the Lrfn family of type I transmembrane glycoproteins (1). Human SALM4 is synthesized as a 628 amino acid (aa) precursor that contains a 16 aa signal sequence, a 523 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 68 aa cytoplasmic region. The ECD consists of six leucine-rich repeats (LRR), an IgC2-like domain, and a fibronectin type-III domain, tandemly aligned in that order (1, 2). In addition, there are five potential sites for N-linked glycosylation. SALM4 and -5 lack a C-terminal intracellular PDZ binding domain, which is conserved among SALMs 1‑3. Mature human SALM4 shares 96% aa sequence identity with mature mouse SALM4. Northern blot analysis showed that in mice, SALM4 is strongly expressed in the adult brain and is also present in the adult gastrointestinal tract and kidneys (1). It is distributed throughout the neuron, including the growth cone (3). In the developing mouse embryo, a temporal expression profile blot revealed a general increment of expression around E10.5, with weak expression detected before E10.5 (1). SALM4, like the other SALMs, promotes neurite outgrowth (3). Specifically, the SALMs modify total outgrowth and neurite branching (3).

Long Name

Leucine-rich Repeat and Fibronectin Type III Domain Containing Protein 3

Alternate Names

FIGLER1, LRFN3

Entrez Gene IDs

79414 (Human); 233067 (Mouse); 308495 (Rat)

Gene Symbol

LRFN3

UniProt

Q9BTN0

Additional SALM4/LRFN3 Products

Product Documents

Product Datasheets

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COA

SDS

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Product Specific Notices for Human SALM4/LRFN3 Alexa Fluor® 647-conjugated Antibody

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

Citations
Reviews

Human SALM4/LRFN3 Alexa Fluor® 647-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5349R

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity