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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)

Antibody Source

Polyclonal Sheep IgG

Product Specifications

Immunogen

E. coli-derived recombinant human PIAS2
Met1-Gly550
Accession # O75928

Specificity

Detects human PIAS2 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human PIAS2 Alexa Fluor® 405-conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PIAS2

PIAS2 (Protein inhibitor of activated STAT2; also DIP, MIZ1 and PIASx-beta) is a 45‑70 kDa member of the PIAS family of molecules. In primary cells and tissues it is expressed in T and NK cells, and in testis where it likely regulates spermatogenesis. However, it is widely expressed in cultured cell lines. PIAS2 is known to inhibit STAT4-mediated gene activation, and to interact with inactive ELK‑1, promoting its activation via HDAC2 dissociation and desumoylation. Human PIAS2 is 621 amino acids (aa) in length. It contains a SAP domain (aa 11‑45), a zinc-finger region (aa 331‑408), a SUMO-binding motif (aa 467‑472) and an NLS (aa 484‑492). There are multiple splice variants. PIAS2x-beta /ARIP3 shows a 22 aa substitution for aa 551‑621, while PIAS-NY shows a 12 aa substitution for aa 1‑8, followed by a truncation after Gly401. Two others show a four aa substitution for aa 504‑621, and an alternate start site at Met10. Over aa 1‑550, human PIAS2 shares 98% aa identity with mouse PIAS2.

Long Name

Protein Inhibitor of Activated STAT 2

Alternate Names

ARIP3, DIP, MIZ1, PIASx alpha, SIZ2, ZMIZ4

Entrez Gene IDs

9063 (Human); 17344 (Mouse)

Gene Symbol

PIAS2

UniProt

Additional PIAS2 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human PIAS2 Alexa Fluor® 405-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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