Human Pepsinogen A5/PGA5 Alexa Fluor™ Plus 405-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB8457AFP405
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 974731
Product Specifications
Immunogen
Human embryonic kidney cell, HEK293-derived human Pepsinogen A5/PGA5 protein
Specificity
Detects human Pepsinogen A5/PGA5 protein in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Pepsinogen A5/PGA5
Pepsins are aspartic proteases that are synthesized in the gastric mucosa and secreted into the stomach. They are released as zymogens called pepsinogens which are then converted to active pepsins by the acidic pH of gastric juices (1-3). PGA3, PGA4, and PGA5 are human Pepsinogen A isozymogens that differ in sequence by 24 amino acid (aa) residues (4, 5). This recombinant human Pepsinogen A corresponds to PGA5. Human Pepsinogen A isozymogens share approximately 56% aa sequence identity with mouse and rat Pepsinogen A isozymogens. Pepsins have optimal activity under conditions of acidic pH and are inhibited by pepstatin (6, 7). Pepsin A has broad substrate specificity, but preferentially cleaves peptide bonds involving aromatic and aliphatic amino acids.
References
- Athauda, S.B. et al. (1989) J. Biochem. 106:920.
- Kageyama, T. et al. (1989) J. Biochem. 105:15.
- Kageyama, T. (2002) Cell. Mol. Life Sci. 59:288.
- Zwiers, A. et al. (1994) Clin. Nephrol. 41:153.
- Nakai, H. et al. (1986) Cytogenet. Cell Genet. 43:215.
- Marciniszyn, J., Jr. et al. (1976) J. Biol. Chem. 251:7088.
- Kageyama, T. and K. Takahashi (1980) J. Biochem. 88:571.
Alternate Names
PGA5
Entrez Gene IDs
5222 (Human)
Gene Symbol
PGA5
UniProt
Additional Pepsinogen A5/PGA5 Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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