Detects human NKX6.1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human NKX2E, 2.2, 3.1 or recombinant mouse NKX3.1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human NKX6.1 Antibody

NKX6.1 in betaTC‑6 Mouse Cell Line.

NKX6.1 was detected in immersion fixed betaTC-6 mouse beta cell insulinoma cell line using Human NKX6.1 Monoclonal Antibody (Catalog # MAB5857) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human NKX6.1 Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed betaTC‑6 mouse beta cell insulinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKX6.1

NKX6.1 (NK homeobox factor 6.1; also NKX6A) is a 39-43 kDa member of the homeodomain family of transcription factors. It is expressed in embryonic ventral neural tube where it drives neural progenitors to form motoneurons and V2-type interneurons. It is also expressed in both fetal and neonatal pancreas where it promotes a beta-cell phenotype. NKX6.1 exhibits both transcriptional gene repression (NKX6.2 and Dbx2) and activation (cyclin A2 and B1). When overexpressed, it induces beta-cell mitosis. Human NKX6.1 is 367 amino acids (aa) in length. It contains a repressor region composed of Pro and Ala (aa 136-173), a DNA binding homeobox (aa 236-295) and a Glu:Asp-rich transactivation domain (aa 305-337). Over aa 1-120, human NKX6.1 shares 96% aa identity with mouse NKX6.1.

Long Name

NK6 Homeobox 1

Alternate Names

NKX6-1, NKX6A

Entrez Gene IDs

4825 (Human); 18096 (Mouse); 65193 (Rat)

Gene Symbol

NKX6-1

UniProt

P78426 (Human)

Additional NKX6.1 Products

Product Documents for Human NKX6.1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human NKX6.1 Antibody

For research use only

Citations
Reviews

Human NKX6.1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5857

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all NKX6.1 Antibodies »

Product Specifications

Immunogen

Specificity