Western blot shows lysates of U937 human histiocytic lymphoma cell line and C2C12 mouse myoblast cell line. Gels were loaded with 30 µg of U937 and 40 µg of C2C12 cytoplasmic (Cyto) extracts and 15 µg of U937 and 20 µg of C2C12 nuclear (Nuc) extracts. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse YY1 Monoclonal Antibody (Catalog # MAB3784) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for YY1 at approximately 60-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse YY1 Antibody

Application

Recommended Usage

Western Blot

0.5 µg/mL
Sample: U937 human histiocytic lymphoma cell line and C2C12 mouse myoblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: YY1

YY1 (Yin Yang 1) is a multifunctional transcription factor belonging to the GLI-Kruppel class of zing finger proteins. YY1 is essential for embryogenesis, apoptosis, differentiation, cell growth and proliferation. YY1 binds to the DNA consensus site CGCCATNTT. It can function as an initiator of transcription as well as activating or repressing transcription through protein complexes (e.g. histone deacetylases and histone acetyltransferases).

Long Name

Yin-Yang-1

Alternate Names

DELTA, NF-E1, UCRBP

Entrez Gene IDs

7528 (Human); 22632 (Mouse); 24919 (Rat)

Gene Symbol

YY1

UniProt

P25490

Additional YY1 Products

Product Documents for Human/Mouse YY1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse YY1 Antibody

For research use only

Citations
Reviews

Human/Mouse YY1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3784

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all YY1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse YY1 Antibody

Detection of Human and Mouse YY1 by Western Blot.