Human/Mouse/Rat Thioredoxin-2 Alexa Fluor® 700-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3254N
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse Thioredoxin-2
Thr60-Gly166
Accession # P97493
Thr60-Gly166
Accession # P97493
Specificity
Detects endogenous human, mouse and rat Thioredoxin 2 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Thioredoxin-2
Thioredoxin-2 (Trx2), also known as mitochondrial Thioredoxin, belongs to the evolutionarily conserved Thioredoxin family. These proteins share the Thioredoxin fold containing the active site-CGPC motif. In their reduced form, the active site cysteine residues reduce protein disulfides. The resulting active site disulfide is subsequently reduced in a reaction catalyzed by a NADPH-dependent Thioredoxin reductase. Thioredoxin-2 contains an N-terminal 59 amino acid (aa) transit sequence that is cleaved upon translocation to mitochondria. The amino acid sequence of mature mouse Thioredoxin‑2 is identical to mature rat Thioredoxin-2 and 98% identical to human Thioredoxin-2. Thioredoxin-2 interacts with specific components of the mitochondrial respiratory chain and helps regulate the membrane potential. Thioredoxin-2 is ubiquitously but variably expressed and high expression confers resistance to oxidant‑induced apoptosis.
Alternate Names
MT-TRX, Thioredoxin2, Trx2, TXN2
Gene Symbol
TXN2
UniProt
Additional Thioredoxin-2 Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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