Human/Mouse/Rat PALS1/MPP5 Alexa Fluor® 350-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7979U
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PALS1/MPP5
Ala392-Ser509
Accession # Q8N3R9
Ala392-Ser509
Accession # Q8N3R9
Specificity
Detects human, mouse, and rat PALS1/MPP5 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: PALS1/MPP5
MPP5 (Membrane Palmitoylated Protein 5; also PALS1/Proteins Associated with Lin7 #1) is a 70-80 kDa member of MAGUK (Membrane-Associated Guanylate Kinase) family of proteins. It is ubiquitously expressed, and plays a key role in the creation of cell polarity and adhesion. Although not its only function, MPP5 is essential for tight junction integrity. Crumbs proteins (Crb-1 and -3) are apically-oriented, integral membrane proteins. These transmembrane proteins are known to bind to cytosolic MPP5, which, in turn, links to PATJ, which, in turn, binds to either cytosolic ZO-3, or to 4-transmembrane laterally-embedded claudin-1. Subsequent binding of ZO-3 with actin unite the cytoskeleton with the cell membrane. Human MPP5 is 675 amino acids (aa) in length. It contains a utilized phosphorylation site at Ser25, two consecutive L27 domains (aa 120-177 and 179-235) that bind PATJ, one PDZ domain (aa 256-336) that binds crumb proteins, an SH3 region (aa 345-417), and a C‑terminal guanylate kinase-like domain (aa 479-660). MPP5 forms both homodimers and heterodimers with MPP4. There is one splice variant that utilizes an alternative start site at Met35. Over aa 392-509, human and mouse MPP5 are identical in amino acid sequence. Overall, human and mouse MPP5 share 97% aa sequence identity.
Long Name
Protein Associated with Lin Seven 1
Alternate Names
MPP5, Stardust
Gene Symbol
MPP5
UniProt
Additional PALS1/MPP5 Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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