Skip to main content

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Alexa Fluor 532 (Excitation = 534 nm, Emission = 553 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 565320

Product Specifications

Immunogen

E. coli-derived recombinant human Glutathione Peroxidase 4
Gly74-Phe168
Accession # P36969

Specificity

Detects endogenous human, mouse and rat GPX4 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Glutathione Peroxidase 4/GPX4

Glutathione Peroxidase 4 (GPX4; also known as PHGPx) is a monomeric, 21 kDa member of the glutathione peroxidase family of proteins. It is widely expressed and serves to both protect cell membranes from phospholipid and cholesterol hydroperoxidases, and form an inactive enzyme structural component of the sperm mitochondrial capsule. Human GPX4 is 197 amino acids (aa) in length and contains a mitochondrial targeting sequence (aa 1‑27) and an enzymatically active selenocysteine at Sec73. During sperm maturation, the targeting sequence is cleaved, and GPX4 may form inactive covalently-linked oligomers. There are multiple splice variants. One is ubiquitously expressed, cytoplasmic, and shows an alternate start site at Met28. A second is 226 aa in length, nuclear, and shows an Arg-rich 65 aa substitution for aa 1‑28. Over amino acids 74‑168, human GPX4 shares 93% aa identity with mouse GPX4.

Alternate Names

GPX4, PHGPx, snGPx

Entrez Gene IDs

2879 (Human); 625249 (Mouse); 29328 (Rat)

Gene Symbol

GPX4

UniProt

Additional Glutathione Peroxidase 4/GPX4 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

Loading...
Loading...
Loading...