Human/Mouse Peroxiredoxin 5 Alexa Fluor® 750-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3774S
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Alexa Fluor 750 (Excitation = 749 nm, Emission = 775 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Peroxiredoxin 5
Met1-Leu214
Accession # P30044
Met1-Leu214
Accession # P30044
Specificity
Detects endogenous human and mouse Peroxiredoxin 5 in Western blots. In Western blots, this antibody shows no cross‑reactivity with recombinant human Peroxiredoxin 1, 2, 3, or 6.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Peroxiredoxin 5
Peroxiredoxin-5 (Prx-5 or PRDX5; also AOEB166 and thioredoxin reductase) is a 22 kDa, widely expressed mitochondrial antioxidant enzyme that belongs to the atypical 2-Cys class of the TSA/ahpC family of peroxiredoxins. The precursor molecule is 214 amino acids (aa) in length. It contains an N-terminal 52 aa mitochondrial targeting (signal) sequence and a 162 aa (17 kDa) mature enzyme that shows a nuclear export signal between aa 58‑67, and a peroxisome targeting motif at aa 212‑214. There are two catalytic cysteines, one at Cys100 and a second at Cys204 of the precursor. Prx-5 is a monomer that is found in mitochondria, cytosol, nucleus and peroxisomes. It is known to reduce H2O2 and alkyl hydroperoxides. Prx5 can undergo cleavage after Ala52, generating two splice forms. One shows a deletion of aa 59‑147, while a second shows a deletion of aa 103‑146. Mature human Prx-5 is 78% aa identical to mouse Prx-5, with most differences noted over the first 52 amino acids.
Alternate Names
ACR1, AOEB166, AOPP, PMP20, PRDX5, PRXV
Gene Symbol
PRDX5
UniProt
Additional Peroxiredoxin 5 Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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