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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant human ELF3

Specificity

Detects human and mouse ELF3.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human/Mouse ELF3 Alexa Fluor® 594-conjugated Antibody

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: ELF3

ELF3 (E74‑Like Factor 3; also ESE‑1, ESX, ERT and JEN) is a 41‑43 kDa member of the ETS family of proteins. During uncomplicated (non‑inflammatory) periods of cell differentiation, ELF3 is expressed exclusively by epithelial cells, repressing genes needed during early differentiation, and promoting genes needed for full differentiation. Under conditions of inflammation, cells such as monocytes, endothelial cells and chrondrocytes will express ELF3 and produce molecules such as Ang1 and COX2. Human ELF3 is 371 amino acids (aa) in length. It contains one PNT/pointed dimerization domain (aa 46‑132), a protein stabilizing PEST sequence (aa 210‑225), an A/T Hook region that binds to AT‑rich DNA sequences (aa 236‑252), and an ETS DNA binding domain (aa 273‑355). ELF3 interacts with CREBBP, EP300, KU70 and KU86. There is one splice variant that shows a deletion of aa 174‑200. Over aa 1‑173, human ELF3 shares 87% aa identity with mouse ELF3.

Long Name

E74-like factor 3

Alternate Names

EPR1, ERT, ESX, JEN

Entrez Gene IDs

1999 (Human); 13710 (Mouse); 304815 (Rat)

Gene Symbol

ELF3

Additional ELF3 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse ELF3 Alexa Fluor® 594-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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