Detects human Methionine Aminopeptidase 1/METAP1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human Methionine Aminopeptidase 2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Applications for Human Methionine Aminopeptidase 1/METAP1 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Methionine Aminopeptidase 1 (Catalog # 3537-ZN)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Methionine Aminopeptidase 1/METAP1

The human METAP1 gene encodes Methionine Aminopeptidase 1, a member of the M24 family of metalloproteases. METAPs catalyze the removal of the initiator methionine residue from nascent peptides (1) and are essential for cell growth (2). Inhibition of METAPs provides a novel strategy in developing anti-cancer drugs (3). The amino acid sequence of human METAP1 is 99%, 98%, 95% and 64% identical to that of chimpanzee, rat/mouse, chicken and fruit fly.

References

Lowther, W.T. and B.W. Matthews (2000) Biochim. Biophys. Acta. 1477:157.
Li, X. and Y.H. Chang (1995) Proc. Natl. Acad. Sci. USA 92:12357.
Liu, S. et al. (1998) Science 282:1324.

Alternate Names

MAP1, MetAP1

Entrez Gene IDs

23173 (Human); 75624 (Mouse)

Gene Symbol

METAP1

UniProt

P53582

Additional Methionine Aminopeptidase 1/METAP1 Products

Product Documents for Human Methionine Aminopeptidase 1/METAP1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Methionine Aminopeptidase 1/METAP1 Antibody

For research use only

Citations
Reviews

Human Methionine Aminopeptidase 1/METAP1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3537

Key Product Details

Species Reactivity

Validated:

Cited:

Applications