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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant human Lipase I
Arg14-His154
Accession # Q6XZB0

Specificity

Detects human Lipase I in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Lipase I Alexa Fluor® 594-conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Lipase I

Lipase I (Lipase member I; also mPA‑PLA1 beta LPDL and PLA1C) is a 55 kDa member of the PLA1 subfamily, pancreatic lipase family, AB hydrolase superfamily of enzymes. It is expressed by sperm, where it is apparently secreted and subsequently binds to membrane-associated HSPGs. Lipase I hydrolyzes phosphatidic acid to generate 2‑acyl‑LPA, which then activates one of four G-protein coupled receptors. Human Lipase I precursor is 460 amino acids (aa) in length. It contains a 15 aa signal sequence, followed by an active catalytic site composed of Ser159, Asp183 and His268. Lipase I is known to be phosphorylated on Ser25. There are two isoform variants, both of which show a 34 aa substitution for aa 1‑13, and only one of which shows an eight aa substitution for aa 245‑460. Over aa 14‑154, human Lipase I shares 61% aa identity with mouse Lipase I.

Alternate Names

CT17, LIPI, LPDL, PRED5

Entrez Gene IDs

149998 (Human); 288322 (Rat)

Gene Symbol

LIPI

UniProt

Additional Lipase I Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Lipase I Alexa Fluor® 594-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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