Human HPRG Alexa Fluor® 405-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1869V
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human HPRG
Val19-Lys525
Accession # P04196
Val19-Lys525
Accession # P04196
Specificity
Detects human HPRG in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant mouse HPRG is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human HPRG Alexa Fluor® 405-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: HPRG
Human histidine‑rich glycoprotein (HPRG) is a multidomain, monomeric, secreted, 67‑75 kDa member of the cystatin superfamily of molecules (1, 2). Its name derives from the fact that 26% of its amino acids (aa) are histidine and proline. In human, it is synthesized as a 525 aa precursor that contains an 18 aa signal sequence and a 507 aa mature region (3). Five distinct domains are recognized in the mature molecule. There are two N‑terminal cystatin‑like modules (aa 19‑254) and one His‑Pro‑rich region (aa 350‑497) that is flanked by two Pro‑rich segments (aa 276‑321 and 498‑525) (3, 4). The His‑Pro‑rich region contains 10 tandem repeats with an HHPHG motif, and the N‑ and C‑termini are linked by a disulfide bond (3, 5, 6). Human HPRG is only 60% aa identical to mouse HPRG. There are multiple ligands for HPRG. These include small molecular weight molecules (metal ions; heme), hemostatic molecules (heparan sulfate; TSP; plasminogen), and immune system components (T cells; macrophages) (1, 5). About 50% of plasma plasminogen circulates bound to HPRG. Upon immobilization to cell surface tropomyosin in a Zn++‑dependent manner, it is converted to plasmin by tPA (7‑9). HPRG also shows antiangiogenic activity on endothelial cells (10).
Long Name
Histidine-rich Glycoprotein Precusor
Alternate Names
HRG
Entrez Gene IDs
3273 (Human)
Gene Symbol
HRG
UniProt
Additional HPRG Products
Product Documents for Human HPRG Alexa Fluor® 405-conjugated Antibody
Product Specific Notices for Human HPRG Alexa Fluor® 405-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
Loading...
Loading...
Loading...