Human FoxK1 Alexa Fluor® 647-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6366R
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human FoxK1
Lys493-Lys670
Accession # P85037
Lys493-Lys670
Accession # P85037
Specificity
Detects human FoxK1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse FoxK1 is observed and less than 5% cross-reactivity with recombinant human (rh) FoxK2 and rhFoxJ1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human FoxK1 Alexa Fluor® 647-conjugated Antibody
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: FoxK1
FOX K1 (Forkhead box protein K1; also MNF) is a 75 kDa (predicted) nuclear protein that is a Class 1 member of the FOX family of transcription factors. It should not be confused with FOXK2/ILF1 that has also been referred to as FOXK1. It is widely expressed, but has become known as a marker for skeletal muscle satellite cells in the adult. Human FOXK1 is 733 amino acids (aa) in length. It contains one FHA domain that binds phosphopeptides (aa 116-203), plus a forkhead DNA binding region (aa 305-400) and over a dozen Ser/Thr phosphorylation sites. There are multiple potential splice variants. Two utilize an alternative start site at Met118 that may be accompanied by a 28 aa substitution for aa 673-733, while another contains 24 aa substitution for aa 1-187. Over aa 493-670, human FOXK1 shares 90% aa identity with mouse FOXK1.
Long Name
Forkhead Box K1
Alternate Names
MNF
Gene Symbol
FOXK1
UniProt
Additional FoxK1 Products
Product Specific Notices for Human FoxK1 Alexa Fluor® 647-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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