Detects human Enteropeptidase/Enterokinase in direct ELISAs and Western blots. It recognizes all EK forms containing the heavy chain including the single chain precursor and the mature heterodimer consisting of a heavy and light chain. In Western blots, less than 5% cross‑reactivity with recombinant human (rh) Factor X and rhThrombin is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Applications for Human Enteropeptidase/Enterokinase Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Enteropeptidase/Enterokinase (Catalog # 1585-SE), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Enteropeptidase/Enterokinase (Catalog # 1585-SE) under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Enteropeptidase/Enterokinase

Enterokinase (EK), also called enteropeptidase and TMPRSS15 (gene name), initiates activation of pancreatic proteases by converting trypsinogen to trypsin, which in turn activates chymotrypsin, carboxypeptidases and elastases. Located in intestinal brush border, it is a disulfide bond-linked dimer of the heavy and light chains, which are derived from the same single-chain precursor. The heavy chain consists of a short cytoplamic tail, a transmembrane, a SEA, a SRCR, a MAM, two CUB and two LDL-receptor class A domains. The light chain contains the catalytic domain of trypsin-like serine proteases.

Alternate Names

Enterokinase, ENTK, PRSS7, TMPRSS15

Entrez Gene IDs

5651 (Human)

Gene Symbol

TMPRSS15

UniProt

NP_002763

Additional Enteropeptidase/Enterokinase Products

Product Documents for Human Enteropeptidase/Enterokinase Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Enteropeptidase/Enterokinase Antibody

For research use only

Citations
Reviews

Human Enteropeptidase/Enterokinase Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1585

Key Product Details

Species Reactivity

Validated:

Cited:

Applications