Human Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Alexa Fluor™ Plus 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6530AFP488
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Dimethylarginine Dimethylaminohydrolase 1/DDAH1
Specificity
Detects human Dimethylarginine Dimethylaminohydrolase 1/DDAH1 in direct ELISAs.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Dimethylarginine Dimethylaminohydrolase 1/DDAH1
Dimethylarginine Dimethylaminohydrolase (DDAH) metabolizes asymmetric dimethyl arginine (ADMA) to L-citrulline and dimethylamine, and NG-monomethyl arginine (MMA) to L-citrulline and monomethylamine (1). Two members of the DDAH family have been identified in humans. DDAH1 is widely expressed, especially in liver and kidney. DDAH2 predominates in vascular endothelium and expressed selectively in kidney (2). It is also expressed in immune tissues including spleen, thymus, peripheral leukocytes, lymph nodes, and bone marrow. Over 90 % of endogenous ADMA is metabolized by DDAH with the remainder excreted (3). ADMA and MMA are endogenous inhibitors of nitric oxide synthase (NOS). Thus, enzymes of the DDAH family play a key role in vascular function through the turnover of methylated arginine (4). It has been observed that genetic variation in the DDAH1 and DDAH2 genes is significantly associated with serum ADMA levels (5).
References
- Tadashi, O. et al. (1989) J. Biol. Chem. 264:10205.
- Tran, C. T. et al. (2000) Genomics 68:101.
- Tran, C. T. et al. (2003) Atheroscler. Suppl. 4:33.
- Leiper, J. et al. (2007) Nat. Med. 13:198.
- Abhary, S. et al. (2010) PNAS PLoS One. 5:e9462.
Alternate Names
DDAHI, Dimethylargininase-1
Entrez Gene IDs
23576 (Human)
Gene Symbol
DDAH1
UniProt
Additional Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Products
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- Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Lysates
- Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Primary Antibodies
- Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Proteins and Enzymes
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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