Human Contactin-3 Alexa Fluor™ Plus 647-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # AF7125AFP647

Catalog #
Availability
Size / Price
Qty
AF7125AFP647-100UG
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Alexa Fluor Plus 647 (Excitation = 658 nm, Emission = 675 nm)

Antibody Source

Polyclonal Sheep IgG

View all Contactin-3 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Contactin-3

Specificity

Detects human Contactin-3 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse Contactin-3 is observed, and less than 5% cross-reactivity with recombinant human (rh) Contactin-4 and rhContactin-5 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Contactin-3

Contactin-3, also known as BIG-1, CNTN3 and PANG, is a 150 kDa member of the TAG/F3 subgroup of Contactin neuronal adhesion proteins (1, 2). Mature human Contactin-3 consists of six immunoglobulin-like domains, a flexible linker region, four fibronectin type III domains, and a GPI anchor (3, 4). Human Contactin-3 shares 92% aa sequence identity with mouse and rat Contactin-3. Contactin-3 is primarily expressed in the brain in discrete regions including the frontal lobe, occipital lobe, cerebellum, amygdala, and hippocampus (5, 6). Alternative splicing in rat may generate a soluble isoform (4). Mouse plasmacytoma cell lines produce multiple transcripts of Contactin-3 due to the presence of IAP proviruses (3). Immobilized Contactin-3 promotes neurite outgrowth from rat hippocampal neurons (4).

References

  1. Shimoda, Y. and K. Watanabe (2009) Cell Adhesion Migration 3:64.
  2. Denisenko-Nehrbass, N. et al. (2002) J. Physiol. 96:99.
  3. Connelly, M.A. et al. (1994) Proc. Natl. Acad. Sci. 91:1337.
  4. Yoshihara, Y. et al. (1994) Neuron 13:415.
  5. Kamei, Y. et al. (2000) Genomics 69:113.
  6. Yoshihara, Y. et al. (1995) J. Neurobiol. 28:51.

Alternate Names

BIG-1, CNTN3, Contactin3, PANG, PCS

Entrez Gene IDs

5067 (Human); 18488 (Mouse); 54279 (Rat)

Gene Symbol

CNTN3

UniProt

Q9P232

Additional Contactin-3 Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Privacy Policy
Cookie Policy
Site Map
© Copyright 2026 Bio-Techne. All Rights Reserved. All trademarks and registered trademarks are the property of Bio-Techne and its brands unless otherwise specified.