Human Carbohydrate Sulfotransferase 2/
CHST2 Alexa Fluor™ Plus 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5107AFP488
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human CHST2
Specificity
Detects human Carbohydrate Sulfotransferase 2/CHST2 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CHST1, rhCHST5, and recombinant mouse CHST7 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications
Application
Recommended Usage
Immunoprecipitation
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Carbohydrate Sulfotransferase 2/CHST2
The CHST family is comprised of 14 genes in both human and mouse. All members of this family are Golgi-localized type II membrane proteins. Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (1). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (2). Human CHST2, also known as N-acetylglucosamine-6-O-sulfotransferase 1 (GlcNAc6ST-1) and Gal/GalNAc/GlcNAc 6-O-sulfotransferase (GST-2), was previously shown to act on non-reducing GlcNAc residues (3). The enzyme is known to be involved in biosynthesis of L-selectin ligand sialyl 6-sulfo Lewis X (4) and therefore plays a role in lymphocyte homing (5).
References
- Hemmerich, S. and Rosen, S. (2000) Glycobiology 10:849.
- Bowman, K. G. and Bertozzi, C. R. (1999) Chem. Biol. 5:447.
- Sakaguchi, H. et al. (2000) Biochim. Biophys. Acta 1523:269.
- Uchimura, K. et al. (1998) J. Biol. Chem. 273:22577.
- Li, X. et al. (2001) J. Leukoc. Biol. 69:565.
- Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, p.964.
- MacRae, I.J. et al. (2000) Biochemistry 39:1613.
- Wu, Z.L. et al. (2002) FASEB J. 16:539.
Alternate Names
C6ST, CHST2, GlcNAc6ST-1, GN6ST, GST-2
Gene Symbol
CHST2
UniProt
Additional Carbohydrate Sulfotransferase 2/CHST2 Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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