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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Immunocytochemistry, Intracellular Staining by Flow Cytometry, Knockout Validated, Western Blot

Label

Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)

Antibody Source

Polyclonal Sheep IgG

Product Specifications

Immunogen

E. coli-derived recombinant human AHR
Asn704-Leu848
Accession # P35869

Specificity

Detects human AHR in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human AHR Alexa Fluor® 594-conjugated Antibody

Application
Recommended Usage

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Intracellular Staining by Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Knockout Validated

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: AHR

AHR (Aryl-hydrocarbon receptor; also known as bHLHE76) is a 110 kDa member of the bHLH/PAS transcription factor family. It is widely expressed (breast, lung, liver), and serves many functions. First, it binds multiple xenobiotic chemicals in the cytoplasm. This induces dimerization with ARNT, translocation to the nucleus, and activation of P450 genes such as CYP1A1 and UGT1A6. Second, it appears to block cell cycle progression, possibly via a down‑regulation of CDK proteins. And third, it blocks apoptosis by interacting with E2F1, thus silencing TP73 and Apaf1 genes. Human AHR is 848 amino acids (aa) in length. It contains a 10 aa prosegment, plus a 838 aa mature molecule that contains a DNA binding motif (aa 13-40), a bHLH region (aa 41-81), and two PAS domains (aa 111-342). Over aa 704-848, human AHR shares 70% aa identity with mouse AHR.

Long Name

Aryl Hydrocarbon Receptor

Alternate Names

BHLHE76

Entrez Gene IDs

196 (Human); 11622 (Mouse); 25690 (Rat)

Gene Symbol

AHR

UniProt

Additional AHR Products

Product Documents for Human AHR Alexa Fluor® 594-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human AHR Alexa Fluor® 594-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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