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HOXA9 Antibody (9X1L6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15720

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15720-20ul
NBP3-15720-100ul

Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9X1L6 expressed in HEK293

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 173-272 of human HOXA9 (P31269). GAFSENNAENESGGDKPPIDPNNPAANWLHARSTRKKRCPYTKHQTLELEKEFLFNMYLTRDRRYEVARLLNLTERQVKIWFQNRRMKMKKINKDRAKDE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HOXA9 Antibody (9X1L6)

Western Blot: HOXA9 Antibody (9X1L6) [NBP3-15720]

Western Blot: HOXA9 Antibody (9X1L6) [NBP3-15720]

Western Blot: HOXA9 Antibody (9X1L6) [NBP3-15720] - Western blot analysis of extracts of various cell lines, using HOXA9 antibody (NBP3-15720) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry-Paraffin: HOXA9 Antibody (9X1L6) [NBP3-15720]

Immunohistochemistry-Paraffin: HOXA9 Antibody (9X1L6) [NBP3-15720]

Immunohistochemistry-Paraffin: HOXA9 Antibody (9X1L6) [NBP3-15720] - Immunohistochemistry of paraffin-embedded human placenta using HOXA9 Rabbit mAb (NBP3-15720) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for HOXA9 Antibody (9X1L6)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Western Blot

1:1000 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HOXA9

In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters namedA, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated duringembryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcriptionfactor which may regulate gene expression, morphogenesis, and differentiation. This gene is highly similar to theabdominal-B (Abd-B) gene of Drosophila. A specific translocation event which causes a fusion between this gene and theNUP98 gene has been associated with myeloid leukemogenesis. (provided by RefSeq)

Alternate Names

ABD-B, homeo box A9, homeobox A9, Homeobox protein Hox-1G, homeobox protein HOXA9, homeobox protein Hox-A9, homeodomain protein HOXA9, HOX1, HOX1.7, HOX1GMGC1934

Gene Symbol

HOXA9

Additional HOXA9 Products

Product Documents for HOXA9 Antibody (9X1L6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HOXA9 Antibody (9X1L6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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